Author(s):
Sanjita Das, Anupam Dubey, Gyas Khan, Sneha Patnaik, Shivani Shrivastava, Renu Singh
Email(s):
sanjita8@yahoo.co.in , dubeyji963@gmail.com , gkhan@jazanu.edu.sa
DOI:
10.52711/0974-360X.2026.00415 
Address:
Sanjita Das1, Anupam Dubey2, Gyas Khan3, Sneha Patnaik4, Shivani Shrivastava5, Renu Singh5
1Department of Pharmacy, SMAS, Galgotias University, Plot No. 2, Sector 17-A, Yamuna Expressway, Greater Noida - 201310, Uttar Pradesh, India.
2AnovIP, 5th Floor, Gulmohar House, Yusuf Sarai, Green Park, Delhi - 110049
3Department of Pharmacology and Toxicology, College of Pharmacy, Jazan University, Post Box: 45142, Jazan, KSA, Saudi Arabia.
4Department of Physiotherapy, Jamia Hamdard, Hamdard Nagar, New Delhi
5Accurate College of Pharmacy, Plot No. 49, Knowledge Park-II, Greater Noida, Uttar Pradesh, India.
*Corresponding Author
Published In:
Volume - 19,
Issue - 7,
Year - 2026
ABSTRACT:
As cysteine is a proteolytic enzyme present in Tabernaemonatana divaricate latex, the primary goal of this study was to isolate and determine its nanoemulgel formulation for the management of functional gastrointestinal disorders (FGID) using different experimental animal models. The effect of the formulated nanoemulgel of isolated protease from the latex of T. divaricata (NPLT) at the doses of 500 and 1000 mg/kg on managing FGID was screened using four models: food-related model, anti-diarrheal model, water avoidance stress model, and elevated plus maze. Additionally, serotonin levels were measured using a fluorimeter to analyze the impact of the protease on monoamine levels and correlate its mechanism of action. Based on the results of the foresaid models, it was determined that NPLT have anti-FGID properties at both doses (p<0.0001; p<0.05). Elevated brain serotonin level was postulated to be the reason behind its potentiality against FGID. The study showed that the novel formulation nanoemulgel of the proteases from plant T. divaricate latex can help FGID patients by stimulating serotonin release in brain cells.
Cite this article:
Sanjita Das, Anupam Dubey, Gyas Khan, Sneha Patnaik, Shivani Shrivastava, Renu Singh. Formulation and Evaluation of Nanoemulgel of Proteases from Tabernaemonatna divaricata Latex for Treating FGID. Research Journal of Pharmacy and Technology. 2026;19(7):2909-6. doi: 10.52711/0974-360X.2026.00415
Cite(Electronic):
Sanjita Das, Anupam Dubey, Gyas Khan, Sneha Patnaik, Shivani Shrivastava, Renu Singh. Formulation and Evaluation of Nanoemulgel of Proteases from Tabernaemonatna divaricata Latex for Treating FGID. Research Journal of Pharmacy and Technology. 2026;19(7):2909-6. doi: 10.52711/0974-360X.2026.00415 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2026-19-7-2
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