Author(s):
Priyadharshini N, Malathi S
Email(s):
malathis@psgpharma.ac.in
DOI:
10.52711/0974-360X.2026.00502 
Address:
Priyadharshini N1, Malathi S2*
1The Tamil Nadu Dr. M.G.R Medical University, Guindy, Chennai – 600032, Tamil Nadu, India.
2Department of Pharmaceutical Analysis, PSG College of Pharmacy, Coimbatore - 641004, Tamil Nadu, India.
*Corresponding Author
Published In:
Volume - 19,
Issue - 8,
Year - 2026
ABSTRACT:
This study presents the development of facile, robust analytical methodologies: RP-HPLC and HPTLC methods for simultaneously determining Flunarizine Dihydrochloride and Propranolol Hydrochloride in bulk and fixed-dose combination. For RP-HPLC, separation was achieved using XBridgeTM Phenyl 5µm (4.6 × 250mm) stationary phase employing Methanol as the solvent and binary eluent system consist of Acetonitrile: Methanol (50:50) ratio, delivered at a flow rate of 1 ml/min. Detection occurred at 267nm (Isoabsorptive Point), yielding the retention times of 2.9 minutes for Flunarizine dihydrochloride and 5.26 minutes for Propranolol hydrochloride. The analytes exhibited excellent linearity across the concentration ranges of 2-10mcg/ml for Flunarizine also 8 - 40mcg/ml for Propranolol. The LOD values of Flunarizine and Propranolol determined to be 0.1 and 0.5mcg/ml. The HPTLC method employed a developing solvent of Acetone: Methanol: Chloroform: Toluene (0.5:1:8:0.5) to elute the drugs, with the Rf Value of 0.89 for Flunarizine dihydrochloride and 0.15 for Propranolol hydrochloride. Linearity was observed in the range of 200 - 600ng/Spot for Flunarizine dihydrochloride and 800-2400ng/spot for Propranolol hydrochloride. Detection was carried out using 254nm. LOD of Flunarizine dihydrochloride and Propranolol hydrochloride were beginning 20-400ng/spot. Comprehensive validation, encompassing linearity, LOD, LOQ and recovery studies, sustained the precision, reliability, and analytical performance of both techniques. The outcomes underscore the applicability of the proposed methodologies for routine quality control and quantitative assessment of these analytes in pharmaceutical formulations and bulk substances.
Cite this article:
Priyadharshini N, Malathi S. Development and Validation of Integrated RP-HPLC and HPTLC Strategies for the Concurrent Estimation of Flunarizine Dihydrochloride and Propranolol Hydrochloride in Pharmaceutical Matrices. Research Journal of Pharmacy and Technology. 2026;19(8):3547-2. doi: 10.52711/0974-360X.2026.00502
Cite(Electronic):
Priyadharshini N, Malathi S. Development and Validation of Integrated RP-HPLC and HPTLC Strategies for the Concurrent Estimation of Flunarizine Dihydrochloride and Propranolol Hydrochloride in Pharmaceutical Matrices. Research Journal of Pharmacy and Technology. 2026;19(8):3547-2. doi: 10.52711/0974-360X.2026.00502 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2026-19-8-19
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