Author(s):
Ivan O. Surzhikov, Vasyl A. Chornyi, Volodymyr A. Mishchenko, Victoriya A. Georgiyants
Email(s):
vgeor@nuph.edu.ua
DOI:
10.52711/0974-360X.2026.00511 
Address:
Ivan O. Surzhikov1, Vasyl A. Chornyi1,2, Volodymyr A. Mishchenko1, Victoriya A. Georgiyants1*
1National University of Pharmacy, Kharkiv, Ukraine
2JSC Farmak, Kyiv, Ukraine.
*Corresponding Author
Published In:
Volume - 19,
Issue - 8,
Year - 2026
ABSTRACT:
This study aimed at developing and validating a high-performance liquid chromatography (HPLC) procedure to assay of ibuprofen, caffeine, methylparaben and propylparaben in a fixed-combinated syrup. Method was optimized in terms of the composition of mobile phase, wavelengths of the detection and chromatographic conditions to allow successful resolution of the entire analytes. The chromatographic separation was performed on a Zorbax SB-C18 column (150 × 4.6mm, 5µm) using gradient elution with a mobile phase consisting of potassium dihydrogen phosphate buffer (pH 2.2) and acetonitrile. The flow rate was 1.0mL/min, the column temperature was maintained at 25°C, and detection was carried out at 210nm, 254nm, and 273nm depending on the analyte. Method validation was carried out in compliance with the terms of the State Pharmacopoeia of the Ukraine and ICH Q2(R2), which involved such parameters as specificity, linearity, accuracy, precision, and robustness. This method was very selective and linear in the confidence interval of 70% to a 130 percent concentration; it showed good accuracy (98.0 to 102.0 percent). The procedure was also resistant to slight changes in the analyses, and intra- and inter-day accuracy were found to be within the range of the acceptable (%RSD <2.0). The approach proposed is applicable to the quality control of the multicomponent drug syrups in routine procedures.
Cite this article:
Ivan O. Surzhikov, Vasyl A. Chornyi, Volodymyr A. Mishchenko, Victoriya A. Georgiyants. Simultaneous Determination of Ibuprofen, Caffeine and Preservatives in Combined Sirup. Research Journal of Pharmacy and Technology. 2026;19(8):3617-4. doi: 10.52711/0974-360X.2026.00511
Cite(Electronic):
Ivan O. Surzhikov, Vasyl A. Chornyi, Volodymyr A. Mishchenko, Victoriya A. Georgiyants. Simultaneous Determination of Ibuprofen, Caffeine and Preservatives in Combined Sirup. Research Journal of Pharmacy and Technology. 2026;19(8):3617-4. doi: 10.52711/0974-360X.2026.00511 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2026-19-8-28
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