Author(s):
Chandni Chandarana, Zeal Naik, Ashish Patel, Anjana Bera, Parixit Prajapati
Email(s):
chandnichandarana7343@gmail.com , zeelnaik1200@gmail.com , aashishpatel2388@gmail.com , anjanaabera@gmail.com , parixitprajapati@gmail.com
DOI:
10.52711/0974-360X.2026.00531 
Address:
Chandni Chandarana1, Zeal Naik2, Ashish Patel3, Anjana Bera4, Parixit Prajapati5*
1Assistant Professor, SSR College of Pharmacy, Sayli, Silvassa - 396230, India. Orcid: 0000-0003-3049-0664.
2Research Scholar, SSR College of Pharmacy, Sayli, Silvassa - 396230, India. Orcid: 0009-0009-9752-4911.
3Associate Professor, Pharmaceutical Chemistry, Parul Institute of Pharmacy, Parul University India. Orcid: 0000-0001-5773-3756.
4Associate professor, Aryatej institute of pharmacy, Morbi- India. Orcid: 0000-0001-8140-1014.
5Assistant Professor, SSR College of Pharmacy, Sayli, Silvassa- 396230, India. Orcid: 0000-0001-9323-0179.
*Corresponding Author
Published In:
Volume - 19,
Issue - 8,
Year - 2026
ABSTRACT:
Corchorus depressus exhibits several pharmacological activities, including aphrodisiac, antipyretic, and demulcent effects, and is used in the treatment of gonorrhea. This study aims to systematically standardize Corchorus depressus, focusing on evaluating its standardization parameters, as no comprehensive standardization has been reported to date. The markers Diosgenin and Stigmasterol in the root powder were quantified using a developed and validated HPLC method. Various standardization parameters were assessed, including organoleptic properties, macroscopy, powder microscopy, physicochemical parameters, preliminary phytochemical screening, microbial contamination, and toxicological profiles. The HPLC method that was developed turned out to be straightforward, linear, accurate, precise, and selective. Chromatographic separation was achieved on a Symmetry C18 column using isocratic elution and a mobile phase consisting of acetonitrile and water (98:2, v/v). At 210nm, a UV detector was employed for detection. Diosgenin and stigmasterol had retention periods of 5.6 and 10.2 minutes, respectively. After being validated in compliance with ICH guideline Q2(R1), the method showed satisfactory system suitability characteristics and analytical performance. The root powder of Corchorus depressus was discovered to contain 0.281% w/w of diosgenin and 0.312% w/w of stigmasterol. This approved approach allows for the simultaneous quantification of these phytoconstituents.
Cite this article:
Chandni Chandarana, Zeal Naik, Ashish Patel, Anjana Bera, Parixit Prajapati. Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Diosgenin and Stigmasterol in root powder of Corchorus depressus. Research Journal of Pharmacy and Technology. 2026;19(8):3765-0. doi: 10.52711/0974-360X.2026.00531
Cite(Electronic):
Chandni Chandarana, Zeal Naik, Ashish Patel, Anjana Bera, Parixit Prajapati. Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Diosgenin and Stigmasterol in root powder of Corchorus depressus. Research Journal of Pharmacy and Technology. 2026;19(8):3765-0. doi: 10.52711/0974-360X.2026.00531 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2026-19-8-48
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