Author(s):
Ahyar Ahmad, Abdul Karim, Siti Halimah Larekeng, Harningsih Karim, Siti Roszilawati Binti Ramli
Email(s):
ahyarahmad@gmail.com
DOI:
10.52711/0974-360X.2026.00489 
Address:
Ahyar Ahmad1,2*, Abdul Karim1, Siti Halimah Larekeng3,4,5, Harningsih Karim6, Siti Roszilawati Binti Ramli7
1Department of Chemistry, Faculty of Mathematics and Natural Science, Hasanuddin University, Perintis Kemerdekan KM 10, Makassar City, 90245, South Sulawesi, Indonesia.
2Puslitbang Biopolimer dan Bioproduk, Institute for Research and Community Services (LPPM), Hasanuddin University, Makassar 90245, Indonesia.
3Faculty of Forestry, Hasanuddin University, Jl. Perintis Kemerdekaan KM.10, Tamalanrea, Makassar City, South Sulawesi 90245, Indonesia.
4Research Collaboration Center for KARST Microbes, BRIN-LPPM, Hasanuddin University, Jl. Perintis Kemerdekaan KM.10, Tamalanrea, Makassar City, South Sulawesi 90245, Indonesia.
5KARST Bioprospecting and Society Research Group, Hasanuddin University, Jl. Perintis Kemerdekaan Km. 10, Makassar 90245, Indonesia.
6Department of Pharmacy, School of Pharmacy YAMASI, Jl. Mapala 2 Blok D5 No. 10, Makassar 90222, Indonesia.
7Bacteriolog
Published In:
Volume - 19,
Issue - 8,
Year - 2026
ABSTRACT:
L-methioninase has recently gained significant attention, particularly for its potential as an anticancer agent. The biodiversity of marine organisms, particularly the red alga Eucheuma spinosum, holds promising potential as a source of anticancer compounds, as supported by toxicity data from several previous studies. This research aimed to isolate the L-methioninase enzyme from symbiotic bacteria of the red macroalga Eucheuma spinosum collected from Pajukukang District, Bantaeng, Indonesia, for use as an anticancer and antioxidant agent. Screening and identification of bacterial isolates revealed the species Providencia stuartii strain ES 2 (1). Fractionation using ammonium sulfate revealed the highest enzyme activity in fraction F3 (60–80%), with 26.6524U/mL and a specific activity of 7.2326U/mg. The optimal L-methioninase enzyme activity was observed at pH 7, 40°C, and 30 minutes of incubation time. Metal ions such as Ca²?, Mg²?, and Co²? acted as enzyme activators. The brine shrimp lethality test (BSLT) using Artemia salina Leach shrimp larvae and antioxidant tests by the DPPH method yielded an LC50 value of 149.23ppm and an IC50 value of more than 1800ppm. This classifies it as toxic and indicates strong potential for anticancer applications, but not as an antioxidant material.
Cite this article:
Ahyar Ahmad, Abdul Karim, Siti Halimah Larekeng, Harningsih Karim, Siti Roszilawati Binti Ramli. Characterization and In Vitro Study of L-Methioninase from Red Algae Symbiont Bacteria Eucheuma spinosum as Anticancer and Antioxidant Materials. Research Journal of Pharmacy and Technology. 2026;19(8):3445-3. doi: 10.52711/0974-360X.2026.00489
Cite(Electronic):
Ahyar Ahmad, Abdul Karim, Siti Halimah Larekeng, Harningsih Karim, Siti Roszilawati Binti Ramli. Characterization and In Vitro Study of L-Methioninase from Red Algae Symbiont Bacteria Eucheuma spinosum as Anticancer and Antioxidant Materials. Research Journal of Pharmacy and Technology. 2026;19(8):3445-3. doi: 10.52711/0974-360X.2026.00489 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2026-19-8-6
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