Author(s):
Wisnu Prabowo, Sri Sulistyowati, Soetrisno, Ida Nurwati, Paramasari Dirgahayu
Email(s):
wisnu.fetomaternal@staff.uns.ac.id
DOI:
10.52711/0974-360X.2026.00593 
Address:
Wisnu Prabowo1,2*, Sri Sulistyowati2, Soetrisno1, Ida Nurwati3, Paramasari Dirgahayu1,4
1Doctoral Program of Medical Sciences Department, Faculty of Medicine, Universitas Sebelas Maret, Surakarta 57126, Central Java, Indonesia.
2Obstetrics and Gynaecology Department, Faculty of Medicine, Universitas Sebelas Maret, Surakarta 57126, Central Java, Indonesia.
3Department of Biochemistry, Faculty of Medicine, Universitas Sebelas Maret, Surakarta 57126, Central Java, Indonesia.
4Department of Parasitology and Mycology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta 57126, Central Java, Indonesia.
*Corresponding Author
Published In:
Volume - 19,
Issue - 9,
Year - 2026
ABSTRACT:
Preeclampsia is a complex pregnancy disorder that risks both for maternal and neonatal health. The severity is significantly influenced by inflammation. Drugs still use as the major treatment, though, natural product potentially possess therapeutic effect with less side effect. This study conducted to investigate the potency of ethanolic extract of propolis (EEP) as anti-inflammatory in preeclampsia, specifically on IL-12 and E-selectin expression as key inflammatory marker in preeclampsia. In this study, specific phytochemicals CAPE and quercetin in EEP were identified and the potency of EEP on preeclampsia was investigated by in vivo and in silico. Three months old female Swiss mice were induced with 5 IU Pregnant Male Serum Gonadotropin (PMSG) followed by 5 IU Human Chorionic Gonadotropin (hCG). Preeclamptic model was generated by 10 ng anti-Qa injection for 4 days. The EEP at various concentrations (380, 500, and 1000 mg/kg/day) were administered from gestational day 6th to 15th to preeclamptic mice. On day 16, placenta was obtained for immunohistochemistry analysis. Identification of bioactive compounds shows CAPE and quercetin in the EEP. Generally, treatment of EEP effectively mitigated placental inflammation in preeclamptic mice, recovering the trophoblastic cells to a similar condition of non-preeclamptic mice. Administration of 500mg/kg dose EEP (2.58±0.35) is the most significant to reduce IL-12 expression in placenta trophoblastic cell (p<0.05). While the significant decreased of E-selectin observed in administration of 1000mg/kg dose EEP (2.20±0.74). Molecular docking revealed the different binding sites of CAPE and quercetin toward IL-12 and E-selectin, indicating the more potent inhibitory activity of compounds as complex than single compound. Our findings demonstrate that EEP can reduce the expression of key inflammatory proteins in preeclampsia. It highlights the potency of EEP as natural treatment and therapeutic agent for preeclampsia.
Cite this article:
Wisnu Prabowo, Sri Sulistyowati, Soetrisno, Ida Nurwati, Paramasari Dirgahayu. Therapeutic Potential of Ethanolic Extract Propolis (EEP) in Preeclampsia by Downregulating IL-12 and E-Selectin. Research Journal Pharmacy and Technology. 2026;19(9):4253-0. doi: 10.52711/0974-360X.2026.00593
Cite(Electronic):
Wisnu Prabowo, Sri Sulistyowati, Soetrisno, Ida Nurwati, Paramasari Dirgahayu. Therapeutic Potential of Ethanolic Extract Propolis (EEP) in Preeclampsia by Downregulating IL-12 and E-Selectin. Research Journal Pharmacy and Technology. 2026;19(9):4253-0. doi: 10.52711/0974-360X.2026.00593 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2026-19-9-39
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