Author(s):
Yudi Purnomo, Djoko W Soeatmadji, Sutiman B Sumitro, M. Aris Widodo
Email(s):
y_purnomo92@yahoo.com or yudi.purnomo@unisma.ac.id
DOI:
10.52711/0974-360X.2023.00050 
Address:
Yudi Purnomo1*, Djoko W Soeatmadji2, Sutiman B Sumitro3, M. Aris Widodo4
1Pharmacy Department, Faculty of Medicine, University of Islam Malang, Malang, Indonesia.
2Internal Department, Faculty of Medicine University of Brawijaya, Malang, Indonesia.
3Biology Department, Faculty of Life Science, University of Brawijaya, Malang, Indonesia.
4Pharmacology Department, Faculty of Medicine, University of Brawijaya, Malang, Indonesia.
*Corresponding Author
Published In:
Volume - 16,
Issue - 1,
Year - 2023
ABSTRACT:
Incretin hormone is a new therapy for type 2 diabetes related to their activity for regulating blood glucose level. Even though, this hormone is destroyed by Dipeptidyl Peptidase-4 (DPP-4) become in-active form. DPP-4 inhibition will be beneficial for type 2 diabetes therapy. Empirically, some of local herbs are used by Indonesian people to cure diabetes, however, activity on DPP-4 inhibitory has not been examined. The objective of study to examine Indonesian anti-diabetic herbs Urena lobata (U. lobata), Tithonia diversifolia (T. diversifolia) and Carica papaya (C. papaya) through inhibition of DPP-4. The in vitro study use vildagliptin and Gly-pro-p-nitroanilide (GPPN) as a reference standard and substrate respectively. C. papaya leaf extract, T. diversifolia and U. lobata were prepared both of by decoction methods and digeration with ethanol. The data were expressed as mean ± SD and the Inhibitory Concentration (IC)-50 value was calculated by non-linear regression curve fit. U.lobata leaves in ethanolic extract had more inhibitory potency on DPP-4 compare to T. diversifolia and C. papaya with an IC-50 value of 1.65mg/mL, 26.12mg/mL and 34.02mg/mL respectively. Meanwhile, on aqueous extract from U.lobata, C. papaya and T. diversifolia with IC-50 value 6.49mg/mL, 14.99mg/mL and 15.39 mg/mL respectively. U.lobata leaf extract most strongly to inhibit DPP-4 activity regulated by active compounds in its extract like stigmasterol, ß-sitosterol and mangiferin. Ethanolic extract of U.lobata leaves more potent than aqueous extract to inhibit DPP-4 activity and both of them showed the most inhibitory activity on DPP-4 among other.
Cite this article:
Yudi Purnomo, Djoko W Soeatmadji, Sutiman B Sumitro, M. Aris Widodo. Dipeptidyl Peptidase-4 Inhibitory Activity of Indonesian Anti-Diabetic Herbs: Carica Papaya, Tithonia diversifolia, Urena lobata. Research Journal of Pharmacy and Technology 2023; 16(1):273-7. doi: 10.52711/0974-360X.2023.00050
Cite(Electronic):
Yudi Purnomo, Djoko W Soeatmadji, Sutiman B Sumitro, M. Aris Widodo. Dipeptidyl Peptidase-4 Inhibitory Activity of Indonesian Anti-Diabetic Herbs: Carica Papaya, Tithonia diversifolia, Urena lobata. Research Journal of Pharmacy and Technology 2023; 16(1):273-7. doi: 10.52711/0974-360X.2023.00050 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2023-16-1-50
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