Author(s):
Sneha K. S., Srikant Natarajan, Karen Boaz, John Ramapuram, Shrikala Baliga, Nidhi Manaktala, Nunna Sai Chitra
Email(s):
srikant.n@manipal.edu
DOI:
10.52711/0974-360X.2023.00072 
Address:
Sneha K. S.1, Srikant Natarajan2*, Karen Boaz3, John Ramapuram4, Shrikala Baliga5, Nidhi Manaktala6, Nunna Sai Chitra7
1Department of Oral Pathology and Microbiology, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education.
2Professor and Head, Department of Oral Pathology and Microbiology, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education.
3Professor, Department of Oral Pathology and Microbiology, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education.
4Professor and Unit Chief, Department of Medicine, Kasturba Medical College and Hospital, Mangalore, Manipal Academy of Higher Education.
5Professor, Department of Microbiology, Kasturba Medical College and Hospital Mangalore, Manipal Academy of Higher Education.
6Associate Professor, Department of Oral Pathology and Microbiology,
Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education.
7Department of Oral
Published In:
Volume - 16,
Issue - 1,
Year - 2023
ABSTRACT:
Introduction: Candidiasis is the most prevalent opportunistic infection in Acquired Immuno deficiency Syndrome (AIDS). The prolonged and/or recurrent treatment of Candidiasis that results in alteration of profile of Candida species necessitates customizing the antifungal therapy regimen. This study aimed to identify the profile of candidal species in HIV-positive children and adults. Further the colonization of these species was assessed for their antimicrobial sensitivity. Materials and methods: Ten ml saliva was collected from HIV-positive children (n=30) and adults (n=40) and 100µL was cultured on CHROMagarTM followed by identification, counting of species after 48 hours, and antimicrobial sensitivity using the automated VITEK®2 antimicrobial susceptibility testing system. Results: More numbers of HIV-positive children than adults exhibited Candida colonization. The predominant species identified was C. albicans either alone or in combination with C. glabrata, C. tropicalis or C. krusei. An increased proportion of C. glabrata was seen in children whereas the adults showed increase in colonization by C. tropicalis. Assessment of antibiotic resistance showed C. krusei and C. glabrata to be resistant to fluconazole and partly resistant towards Amphotericin B. Conclusion: Higher colonization observed in children may be attributed to the relatively less developed immunity and higher viral load. Multidrug treatment regimens may have caused the changing profile of species from C. albicans to non-albicans. Resistance to fluconazole is attributed to a difference in affinity of the target enzyme and active efflux of fluconazole by the organisms.
Cite this article:
Sneha K. S., Srikant Natarajan, Karen Boaz, John Ramapuram, Shrikala Baliga, Nidhi Manaktala, Nunna Sai Chitra. Candida profile in HIV-Positive children needs a Dynamic clinical appraisal: A microbiological Study. Research Journal of Pharmacy and Technology 2023; 16(1):423-8. doi: 10.52711/0974-360X.2023.00072
Cite(Electronic):
Sneha K. S., Srikant Natarajan, Karen Boaz, John Ramapuram, Shrikala Baliga, Nidhi Manaktala, Nunna Sai Chitra. Candida profile in HIV-Positive children needs a Dynamic clinical appraisal: A microbiological Study. Research Journal of Pharmacy and Technology 2023; 16(1):423-8. doi: 10.52711/0974-360X.2023.00072 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2023-16-1-72
REFERENCES:
1. De Arruda Caceres Net al. Opportunistic Infections in AIDS Patients. Archives of Medicine 2015; 7(5:10): 1-17.
2. Sá MS et al. Clinical and laboratory profile of HIV-positive patients at the moment of diagnosis in Bahia, Brazil. Braz J Infect Dis. 2007;11(4):395-8.
3. Ghannoum MA, Rice LB. Antifungal agents: mode of action, mechanisms of resistance, and correlation of these mechanisms with bacterial resistance. ClinMicrobiol Rev. 1999;12(4):501-17.
4. Bailey & Scott. Laboratory Methods in Basic Mycology. In, Forbes BA, Sahm DF, Weissfeld AS (eds). Diagnostic Microbiology, 11th edition. United States of America, Mosby; 2002. pg.786.
5. Pincus DH. Microbial identification using the biomérieux VITEK ®2 System. Encyclopediaof RapidMicrobiological Methods. 1-5.
6. NeetuJangir, SarojBakshi, Nitya Vyas. Isolation and Detection of Aspergillus and Candida Species in Sputum of HIV Positive Patients and It Correlate with CD4. Research J. Pharm. and Tech. 5(6): June 2012; Page 785-788.
7. SaloojeeH, Violari A. HIV infection in children. BMJ : British Medical Journal. 2001;323(7314):670-674.
8. Chandrashekhara, Sandeepkumar O. HIV / AIDS and Children. Int. J. Nur. Edu. and Research. 2020; 8(4):564-568. doi: 10.5958/2454-2660.2020.00124.6
9. Prasanna Guru. E, Gopinath P. Characterization and detection of biofilm among clinical isolates of Candida species by tube method. Research J. Pharm. and Tech 2016; 9(12):2109-2112. doi: 10.5958/0974-360X.2016.00429.7
10. TamizhPaavaiTha, Gopinath P. Differentiation of Candida dubliniensis on CHROMagar and Pal’s agar. Research J. Pharm. and Tech 2016; 9(12):2150-2154. doi: 10.5958/0974-360X.2016.00436.4
11. V. Sangamithra et al. Candida Infections of the Genitourinary Tract. Research J. Pharm. and Tech. 6(10): October 2013; Page 1111-1115.
12. Zagazig Egypt. Comparison of methods used in identification of Candida albicans. Research J. Pharm. and Tech. 2018; 11(3): 1164-1168. doi: 10.5958/0974-360X.2018.00217.2
13. Aravind. N, Gopinath P. Study on Detecting the Adhesion Ability of Candida Species to Exfoliated Epithelial Cells. Research J. Pharm. and Tech 2016; 9(9):1400-1402. doi: 10.5958/0974-360X.2016.00270.5
14. Kothavade RJ et al. Candida tropicalis: its prevalence, pathogenicity and increasing resistance to fluconazole. J Med Microbiol. 2010; 59(8): 873-80.
15. Maheshwari M, Kaur R, Chadha S. Candida species prevalence profile in HIV seropositive patients from a major tertiary care hospital in New Delhi, India. Journal of Pathogens. 2016; 2016: 1-8.
16. Dar MS et al. An in vitro study of antifungal drug susceptibility of Candida species isolated from human immunodeficiency virus seropositive and human immunodeficiency virus seronegative individuals in Lucknow population Uttar Pradesh. J Oral MaxillofacPathol. 2015 May-Aug;19(2):205-11.
17. Enwuru CA et al. Fluconazole resistant opportunistic oro-pharyngeal Candida and non-Candida yeast-like isolates from HIV infected patients attending ARV clinics in Lagos, Nigeria. Afr Health Sci. 2008;8(3):142-8.
18. TerçasALG, et al. Antifungal Drug Susceptibility of Candida Species Isolated from HIV-Positive Patients Recruited at a Public Hospital in São Luís, Maranhão, Brazil. Frontiers in Microbiology. 2017; 8(298): 1-8.
19. Vermes A et al. Flucytosine: a review of its pharmacology, clinical indications, pharmacokinetics, toxicity and drug interactions. JAntimicrobChemother. 2000 Aug;46(2):171-9.
20. Kofla G, Ruhnke M. Pharmacology and metabolism of anidulafungin, caspofungin and micafungin in the treatment of invasive candidosis: review of the literature. Eur J Med Res. 2011;16(4):159-66.
21. Orozco ASet al. Mechanism of fluconazole resistance in Candidakrusei. Antimicrob Agents Chemother. 1998;42(10):2645-9.
22. Charyulu RN, Devi PP, Jose J, Shetty AV. Formulation and evaluation of mucoadhesive oral gel containing miconazole nitrate for oral candidiasis. Research J Pharm. And Tech 2013: 6(11): 1251-57.
23. Vinay Kumar G., Nisha P. Nair, Prasannakumar D.R., Parmesha. A Study to assess the Quality of life of People Living with HIV/AIDS receiving Anti Retroviral Therapy from the selected Anti-Retroviral Therapy centre’s of Mysore. Int. J. Adv. Nur. Management 2(2): April- June, 2014; Page 90-92.
24. Sanjay Singh Chauhan. The HIV/AIDS Epidemic in India. Research J. Science and Tech. 7(2): April-June, 2015; Page 133-138. doi: 10.5958/2349-2988.2015.00018.2.
25. Pramod P. Lonarkar. Analysing the Prevalence of HIV in India and States. Res. J. Humanities and Social Sciences. 2018; 9(2): 383-390. doi: 10.5958/2321-5828.2018.00066.9